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锟絘(ch锟斤拷n)品锟斤拷锟斤拷
锟斤拷锟斤拷司RIPA Buffer锟絤锟斤拷锟节從革拷锟絅锟剿癸拷锟斤拷锟紹(y锟斤拷ng)锟侥硷拷(x锟斤拷)锟斤拷锟酵革拷锟絅锟斤拷锟斤拷锟(d锟斤拷ng)锟斤實(sh锟斤拷)锟絯锟組锟斤拷锟斤拷锟斤拷锟絏,锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷(j锟斤拷ng)锟結(ji锟斤拷)锟斤拷锟斤拷锟終,锟斤拷锟斤拷锟斤拷锟斤拷锟饺ｏ拷锟斤拷锟斤拷取锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷取锟絕锟教猴拷锟轿凤拷锟姐，锟斤拷锟斤拷1小锟絩(sh锟斤拷)锟斤拷(n锟斤拷i)锟斤拷锟,锟斤拷該試锟斤拷锟斤拷取锟侥匡拷锟斤拷锟阶匡拷锟斤拷锟斤拷Western Blot,锟斤拷锟斤拷锟斤拷锟絴(zh锟斤拷)锟接撅拷锟斤拷锟斤拷吖锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷蔚锟斤拷锟斤拷芯锟,锟斤拷锟斤拷锟结蛋锟阶匡拷锟斤拷BCA,锟斤拷Bradford锟饺凤拷锟斤拷锟統锟斤拷锟斤拷锟阶濓拷取锟
锟絘(ch锟斤拷n)品锟斤拷要锟組锟街和冿拷锟斤拷l锟斤拷
1. 锟絘(ch锟斤拷n)品锟斤拷要锟組锟街ｏ拷Tris,锟斤拷NaCl,锟斤拷NP-40锟斤拷SDS,锟斤拷
2. 4锟芥保锟斤拷,锟斤拷一锟斤拷锟斤拷效锟斤拷
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