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植锟斤拷RNAi锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷锟斤拷(w锟斤拷)
RNAi锟斤拷锟絞(sh锟斤拷)锟斤拷锟叫撅拷锟斤拷锟斤拷锟杰碉拷锟斤拷要锟斤拷锟竭★拷锟斤拷原锟斤拷锟角岋拷某锟斤拷锟斤拷知锟斤拷锟斤拷,锟斤拷锟絆(sh锟斤拷)計锟絋锟斤拷(d锟斤拷o)锟斤拷锟侥拷锟絛sRNA,锟斤拷通锟絕锟斤拷锟絤锟街段岋拷(d锟斤拷o)锟诫細锟斤拷锟斤拷C锟絯使锟絘(ch锟斤拷n)锟斤拷锟斤拷锟斤拷效锟斤拷(y锟斤拷ng)锟斤拷锟斤拷號锟斤拷锟斤拷siRNA锟斤拷锟斤拷(d锟斤拷o)锟铰伙拷锟斤拷锟斤拷_水平锟铰斤拷锟斤拷锟斤拷全锟斤拷默,锟斤拷通锟絕锟斤拷锟斤拷锟斤拷锟阶冿拷锟,锟斤拷锟絙锟斤拷該锟斤拷锟斤拷锟杰★拷
1998锟斤拷,锟斤拷F(xi锟斤拷n)ire锟斤拷[1]锟絣(f锟斤拷)锟紽(xi锟斤拷n),锟斤拷锟絕去锟斤拷锟斤拷锟斤拷锟斤拷锟絰RNA锟斤拷锟斤拷锟斤拷锟斤拷_锟斤拷锟斤拷锟斤拷锟絯锟斤拷锟狡傦拷膯锟斤拷RNA锟斤拷锟斤拷染锟絆锟斤拷锟斤拷锟絧锟絉NA锟斤拷dsRNA锟斤拷锟斤拷锟斤拷锟斤拷模锟斤拷锟斤拷l(f锟斤拷)锟紽(xi锟斤拷n)锟节撅拷锟絰锟叫岋拷(d锟斤拷o)锟斤拷dsRNA,锟斤拷mRNA锟斤拷锟紷锟絧锟斤拷,锟斤拷锟斤拷論锟斤拷锟斤拷某锟絅锟紺锟斤拷锟截拷锟斤拷锟狡壞斤拷锟斤拷锟(n锟斤拷i)源mRNA锟斤拷锟斤拷(d锟斤拷o)锟斤拷某锟斤拷锟斤拷锟斤拷锟侥,锟斤拷锟斤拷锟紻(zhu锟斤拷n)錄猴拷锟斤拷锟斤拷默锟斤拷PTGS锟斤拷,锟斤拷锟节达拷锟斤拷r锟铰ｏ拷锟斤拷锟斤拷锟斤拷锟角伙拷锟絊锟侥碉拷锟斤拷锟斤拷锟斤拷锟斤拷锟絜锟斤拷mRNA,锟斤拷锟紷锟絅锟紽(xi锟斤拷n)锟襟被稱锟斤拷RNA锟斤拷锟芥（RNAi锟斤拷,锟斤拷锟叫撅拷锟斤拷锟斤拷RNAi锟紽(xi锟斤拷n)锟斤拷V锟斤拷锟斤拷锟节达拷锟斤拷锟(sh锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷校锟斤拷锟斤拷锟斤拷斜O(ji锟斤拷n)锟截硷拷锟斤拷锟叫拷锟斤拷锟斤拷mRNA,锟斤拷锟斤拷锟絔該锟斤拷锟斤拷锟斤拷_,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷染锟斤拷锟斤拷锟斤拷D(zhu锟斤拷n)锟斤拷锟接碉拷锟斤拷锟矫★拷
锟斤拷锟斤拷锟街诧拷锟斤拷锟斤拷芯锟絉NAi锟侥岋拷锟斤拷锟绞顾拷锟斤拷袌锟斤拷锟斤拷锟紾US锟斤拷默,锟斤拷锟斤拷锟斤拷锟絕锟斤拷锟絰锟,锟斤拷锟斤拷锟絰鏈硷拷dsRNA锟絋锟斤拷(d锟斤拷o)RNAi效锟绞的高碉拷,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絉NAi锟斤拷锟絞(sh锟斤拷)锟斤拷水锟斤拷锟斤拷锟組锟较斤拷,锟斤拷锟絴薹锟斤拷,锟斤拷锟酵菜★拷锟斤拷锟捷硷拷锟睫伙拷锟斤拷,锟斤拷锟窖斤拷(j锟斤拷ng)锟組锟斤拷锟斤拷一些锟斤拷锟斤拷锟斤拷锟斤拷C锟斤拷锟斤拷锟絇(gu锟斤拷n)锟叫撅拷[3],锟斤拷
锟叫撅拷锟斤拷锟斤拷锟斤拷锟斤拷植锟斤拷锟絯锟斤拷,锟斤拷锟斤拷號锟斤拷锟斤拷siRNA锟斤拷通锟絕植锟斤拷木S锟斤拷系锟統(t锟斤拷ng)锟絓輸,锟斤拷锟斤拷目锟斤拷(bi锟斤拷o)锟斤拷锟斤拷锟截拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷头锟斤拷锟斤拷謩e锟斤拷锟斤拷锟(bi锟斤拷o)記锟斤拷锟斤拷锟斤拷(n锟斤拷i)锟斤拷锟接碉拷5锟斤拷锟剿猴拷3锟斤拷锟剿ｏ拷锟斤拷锟斤拷一锟斤拷锟紹锟斤拷一锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟斤拷锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷式锟截忥拷(f锟斤拷)锟斤拷锟斤拷源锟斤拷锟斤拷?q锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷w,锟斤拷锟絋锟斤拷(d锟斤拷o)锟斤拷锟絖锟絣(f锟斤拷)锟紸锟結(ji锟斤拷)锟斤拷(g锟斤拷u)锟斤拷锟紻(zhu锟斤拷n)錄產(ch锟斤拷n)锟斤拷RNA锟斤拷iphRNA锟斤拷锟斤拷锟杰產(ch锟斤拷n)锟斤拷锟斤拷(w锟斤拷n)锟斤拷锟絲锟斤拷锟斤拷RNAi锟紽(xi锟斤拷n)锟斤拷[5],锟斤拷锟斤拷锟絋锟斤拷(d锟斤拷o)目锟侥伙拷锟斤拷锟侥э拷矢锟,锟斤拷锟絤锟斤拷锟皆廣锟斤拷锟斤拷(y锟斤拷ng)锟矫濓拷锟斤拷锟斤拷,锟斤拷
dsRNA锟斤拷iphRNA锟斤拷通锟絕锟斤拷锟斤拷锟斤拷要锟缴種锟斤拷锟斤拷锟斤拷植锟斤：
锟絩(n锟斤拷ng)锟経锟斤拷锟介導(d锟斤拷o)锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)錄筹拷ihpRNA锟侥凤拷式锟截忥拷(f锟斤拷)锟斤拷锟斤拷源锟斤拷锟斤拷锟絋-DNA锟絴(zh锟斤拷)锟斤拷锟斤拷通锟絕锟絩(n锟斤拷ng)锟経锟斤拷锟介導(d锟斤拷o)锟斤拷锟较碉拷植锟斤拷锟斤拷锟組锟斤拷,锟斤拷
锟斤拷锟斤拷锟絋锟斤拷(d锟斤拷o)锟斤拷锟斤拷锟侥拷锟絍IGS锟斤拷,锟斤拷目锟斤拷(bi锟斤拷o)锟斤拷锟斤拷锟斤拷锟斤拷锟诫病锟斤拷锟斤拷锟斤拷锟斤拷锟叫ｏ拷通锟絕锟絩(n锟斤拷ng)锟経锟斤拷锟介導(d锟斤拷o)锟紻(zhu锟斤拷n)锟斤拷ihpRNA锟斤拷锟絖,锟斤拷
pSGRNAi Vector---植锟斤拷RNAi锟絛锟絯****
锟斤拷锟絑锟斤拷锟絖锟窖斤拷(j锟斤拷ng)锟斤拷C锟斤拷植锟斤拷锟叫ｏ拷锟組锟较斤拷,锟斤拷水锟斤拷锟斤拷锟絊锟斤拷锟斤拷,锟斤拷锟絫锟斤拷,锟斤拷锟斤拷锟阶等★拷
锟斤拷锟斤拷(j锟斤拷)目锟侥伙拷锟斤拷锟皆,锟斤拷锟斤拷锟絰锟斤拷(g锟斤拷u)锟斤拷35S锟斤拷Ubi锟斤拷锟斤拷锟接碉拷RNAi锟絛锟絯,锟斤拷
锟斤拷锟斤拷锟絋锟斤拷(d锟斤拷o)锟侥伙拷锟斤拷锟侥拷锟絭irus induced gene silencing锟斤拷VIGS锟斤拷锟斤拷指锟統锟斤拷目锟斤拷(bi锟斤拷o)锟斤拷锟斤拷片锟轿的诧拷锟斤拷锟斤拷染植锟斤拷锟,锟斤拷锟斤拷锟絋锟斤拷(d锟斤拷o)植锟斤拷锟(n锟斤拷i)源锟斤拷锟斤拷锟侥,锟斤拷锟斤拷锟斤拷锟斤拷锟阶冿拷锟斤拷锟斤拷M锟斤拷锟斤拷锟斤拷(j锟斤拷)锟斤拷锟斤拷變锟斤拷锟叫撅拷目锟斤拷(bi锟斤拷o)锟斤拷锟斤拷墓锟斤拷锟,锟斤拷VIGS锟角革拷锟斤拷(j锟斤拷)植锟斤對RNA锟斤拷锟斤拷锟斤拷锟斤拷锟紺锟狡發(f锟斤拷)展锟斤拷锟斤拷锟斤拷一锟絅锟斤拷锟絞(sh锟斤拷),锟斤拷锟斤拷锟(n锟斤拷i)锟节的凤拷锟接伙拷锟紸(ch锟斤拷)锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)錄猴拷锟斤拷锟斤拷默(post-transcript gene silence)锟斤拷锟斤拷锟,锟斤拷VIGS一锟斤拷(j锟斤拷ng)锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷視锟斤拷锟叫撅拷植锟斤拷锟斤拷锟斤拷艿膹锟斤拷锟斤拷锟斤拷锟斤拷锟,锟斤拷锟矫碉拷锟斤拷锟斤拷锟斤拷锟斤拷芯锟斤拷蛷V锟斤拷锟斤拷(y锟斤拷ng)锟矫ｏ拷锟斤拷锟斤拷锟节燂拷锟斤拷,锟斤拷锟斤拷锟窖碉拷植锟斤拷目锟斤拷锟斤拷锟斤拷锟(y锟斤拷ng),锟斤拷锟斤拷锟絃锟絣(f锟斤拷)锟斤拷锟皆硷拷锟斤拷锟絰锟絳(di锟斤拷o)锟截的癸拷锟杰伙拷锟斤拷锟叫撅拷锟斤拷
锟斤拷锟斤拷(y锟斤拷ng)锟斤拷pTRV1锟斤拷pTRV2锟絛锟絯锟斤拷锟斤拷使锟斤拷,锟斤拷锟斤拷锟斤拷植锟斤拷RNAi,锟斤拷
植锟斤拷RNAi锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷锟侥凤拷锟斤拷(w锟斤拷)要锟斤拷
目锟侥伙拷锟斤拷锟斤拷锟叫伙拷锟竭碉拷锟教栵拷锟
锟斤拷要锟侥嗭拷锟斤拷锟接和篩锟絰锟斤拷锟斤拷,锟斤拷
植锟斤拷RNAi锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷锟侥伙拷锟斤拷锟斤拷锟斤拷
锟絆(sh锟斤拷)計锟斤拷锟斤拷,锟斤拷
锟斤拷(g锟斤拷u)锟斤拷siRNA锟給锟絟(hu锟斤拷n)锟結(ji锟斤拷)锟斤拷(g锟斤拷u)锟斤拷
锟斤拷锟斤拷(j锟斤拷)要锟斤拷,锟斤拷锟斤拷(g锟斤拷u)锟斤拷锟絛锟絯,锟斤拷
锟統锟斤拷锟斤拷C锟斤拷
植锟斤拷RNAi锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷锟侥岋拷锟斤拷锟斤拷(n锟斤拷i)锟斤拷
RNAi锟絴(zh锟斤拷)锟斤拷锟斤拷锟斤拷锟酵撅拷锟斤拷,锟斤拷锟統锟斤拷锟斤拷锟皆旓拷锟斤拷锟斤拷锟斤拷,锟斤拷
锟絣(f锟斤拷)锟斤拷锟侥獻锟斤拷锟絰锟斤拷
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