ELISA锟絲锟統(c锟斤拷)
ELISA锟斤拷Enzyme-Linked Immunosorbnent Assay,锟斤拷ELISA锟斤拷锟斤拷酶聯(li锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷1971锟斤拷锟斤拷Engvall锟斤拷Perlmann锟斤拷(chu锟斤拷ng)锟斤拷锟缴癸拷,锟斤拷锟缴烇拷锟絕锟斤拷锟竭熒癸拷头锟斤拷锟斤拷锟斤拷锟街拷锟斤拷锟揭伙拷锟斤拷锟斤拷卮锟斤拷锟斤拷x锟斤拷锟斤拷锟絟(yu锟斤拷n)影響碉拷锟斤拷锟竭硷拷锟絞(sh锟斤拷)锟斤拷ELISA锟斤拷锟絞(sh锟斤拷)锟斤拷锟矫碉拷锟阶和聚憋拷锟斤拷烯锟斤拷锟斤拷锟斤拷水锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷原-锟斤拷锟絯锟截拷锟皆凤拷锟斤拷(y锟斤拷ng)锟教讹拷锟节癸拷锟斤拷锟絛锟絯锟斤拷锟斤拷锟組(j锟斤拷n)锟斤拷,锟斤拷同锟絩(sh锟斤拷)锟結(ji锟斤拷)锟斤拷酶锟斤拷(du锟斤拷)锟斤拷锟斤拷锟****锟竭伙拷锟斤拷锟矫讹拷锟絘(ch锟斤拷n)锟斤拷锟斤拷锟絗锟斤拷锟饺的檢锟統(c锟斤拷)效锟斤拷锟斤拷
ELISA锟斤拷直锟接凤拷锟斤拷锟絞锟接凤拷,锟斤拷锟紸锟侥凤拷锟斤拷,锟斤拷
锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷Bioo Scientifici锟斤拷R&D System,锟斤拷abcam锟斤拷知锟斤拷锟斤拷司锟斤拷ELISA試锟斤拷锟叫硷拷锟斤拷锟斤拷锟叫發(f锟斤拷)锟絘(ch锟斤拷n)品锟斤拷锟斤拷锟叫ｏ拷锟结供锟斤拷锟絀(y锟斤拷)ELISA锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷(w锟斤拷),锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟侥硷拷锟絞(sh锟斤拷)锟斤拷锟斤拷(w锟斤拷)锟剿員锟絳锟借精湛锟斤拷锟絞(sh锟斤拷)锟斤拷锟絊锟斤拷锟侥斤拷(j锟斤拷ng)锟(y锟斤拷n),锟斤拷锟教時(sh锟斤拷)锟絞锟斤拷(n锟斤拷i)锟斤拷锟斤拷锟斤拷锟紼LISA锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷(ji锟斤拷)省锟絩(sh锟斤拷)锟絞锟酵斤拷(j锟斤拷ng)锟組(f锟斤拷i),锟斤拷锟斤拷邔锟(sh锟斤拷)锟(y锟斤拷n)锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟侥可匡拷锟斤拷,锟斤拷
ELISA锟絲锟統(c锟斤拷)锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1. 锟结供锟斤拷锟絲锟統(c锟斤拷)锟斤拷品锟斤拷锟秸硷拷锟斤拷(bi锟斤拷o)锟斤拷前锟斤拷锟斤拷锟斤拷要锟絲锟統(c锟斤拷)锟侥成凤拷锟角凤拷锟斤拷锟(w锟斤拷n)锟斤拷,锟斤拷
a. 锟絲锟統(c锟斤拷)锟侥樣憋拷锟介細(x锟斤拷)锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷,锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷锟斤拷液锟斤拷锟剿和勶拷(d锟斤拷ng)锟斤拷锟窖拷锟,锟斤拷血锟絳,锟斤拷锟組锟斤拷锟饺★拷
b. 锟接憋拷锟秸硷拷锟斤拷(y锟斤拷ng)锟斤拷锟斤拷-20锟斤拷锟斤拷,锟斤拷锟斤拷锟絃(zh锟斤拷ng)锟节憋拷锟芥應(y锟斤拷ng)-70,锟斤拷
c. 锟接憋拷锟斤拷要锟斤拷一锟斤拷(g锟斤拷)指锟斤拷(bi锟斤拷o)锟斤拷锟斤拷(f锟斤拷)锟阶檢锟統(c锟斤拷)锟斤拷(y锟斤拷ng)锟斤拷锟斤拷锟斤拷20锟斤拷l锟斤拷锟斤拷锟轿孔檢锟統(c锟斤拷)锟斤拷(y锟斤拷ng)锟斤拷锟斤拷锟斤拷10锟斤拷l,锟斤拷锟斤拷锟絟锟斤拷锟接憋拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷峁500锟斤拷l锟斤拷锟斤拷,锟斤拷
d. 锟斤拷(qi锟斤拷ng)锟斤拷锟斤拷锟絔锟酵戯拷锟斤拷取锟斤拷前锟斤拷锟絚锟揭癸拷司锟絅(xi锟斤拷o)锟桔硷拷锟絞(sh锟斤拷)锟剿員锟斤拷通锟斤拷前锟节碉拷取锟斤拷直锟斤拷影響碉拷锟斤拷锟侥岋拷(sh锟斤拷)锟(y锟斤拷n)锟結(ji锟斤拷)锟斤拷,锟斤拷 锟組锟斤拷锟斤拷詳锟斤拷(x锟斤拷)锟侥憋拷锟斤拷锟結锟斤拷,锟斤拷
2.锟絲锟統(c锟斤拷)試锟斤拷锟叫ｏ拷锟斤拷锟斤拷锟斤拷Z锟斤拷锟絖(d锟斤拷)購(g锟斤拷u)锟絀(m锟斤拷i)锟絲锟統(c锟斤拷)試锟斤拷锟叫ｏ拷锟斤拷
ELISA锟絲锟統(c锟斤拷)锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1. 锟斤拷锟斤拷ELISA锟斤拷锟接伙拷使锟斤拷锟斤拷品锟斤拷ELISA锟斤拷,锟斤拷
2. 锟接达拷锟絲锟斤拷品锟斤拷使之锟絚锟斤拷锟洁抗锟絯/锟斤拷原锟斤拷锟斤拷(y锟斤拷ng)一锟轿時(sh锟斤拷)锟斤拷(w锟斤拷n),锟斤拷讓锟斤拷(bi锟斤拷o)锟斤拷锟叫的匡拷原/锟斤拷锟絯锟絚锟斤拷锟斤拷锟絛锟絯锟较的匡拷锟絯/锟斤拷原锟結(ji锟斤拷)锟较ｏ拷锟轿成癸拷锟斤拷锟斤拷(f锟斤拷)锟斤拷锟斤拷,锟斤拷
3. 锟斤拷酶锟斤拷(bi锟斤拷o)锟斤拷锟絯锟斤拷使锟斤拷锟斤拷锟斤拷(f锟斤拷)锟斤拷锟斤拷锟絚酶锟斤拷(bi锟斤拷o)锟斤拷锟絯锟結(ji锟斤拷)锟斤拷,锟斤拷
4. 锟接碉拷锟斤：锟紸锟斤拷式锟斤拷(f锟斤拷)锟斤拷锟斤拷锟叫碉拷酶锟竭伙拷锟斤拷锟斤拷蔀锟斤拷锟缴拷a(ch锟斤拷n)锟斤。锟斤拷锟斤拷(j锟斤拷)锟缴拷锟斤拷锟(y锟斤拷ng)锟侥程讹拷锟組(j锟斤拷n)锟叫匡拷原/锟斤拷锟絯锟侥讹拷锟皆伙拷锟斤拷,锟斤拷
ELISA锟絲锟統(c锟斤拷)锟斤拷锟斤拷(w锟斤拷)锟斤拷(b锟斤拷o)锟斤拷锟(n锟斤拷i)锟斤拷
詳锟斤拷(x锟斤拷)锟侥岋拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷锟紼,锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷試锟斤拷,锟斤拷锟絰锟斤拷,锟斤拷锟斤拷(bi锟斤拷o)锟斤拷(zh锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷锟斤拷锟斤拷
 ELISA锟絲锟統(c锟斤拷)锟斤拷锟斤拷(w锟斤拷)锟絝(shu锟斤拷)锟斤拷
锟酵戯拷锟侥樣憋拷锟秸硷拷锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷要锟襟保达拷,锟斤拷锟叫忌凤拷锟斤拷(f锟斤拷)锟斤拷锟节★拷锟斤拷乜蛻锟斤拷]锟斤拷锟斤拷要锟矫干憋拷锟絓(y锟斤拷n)輸,锟斤拷锟絔锟斤拷前請(q锟斤拷ng)锟絚锟斤拷锟絞(sh锟斤拷)支锟街滐拷通锟絖锟絁(r锟斤拷n),锟斤拷
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