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IP 锟斤拷Co-IP
锟斤拷锟竭筹拷锟斤拷锟斤拷锟斤拷锟矫匡拷原锟斤拷锟絯锟截拷锟皆凤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷目锟侥碉拷锟阶碉拷一锟絅锟斤拷锟斤拷,锟斤拷锟斤拷锟絯锟絚锟斤拷锟斤拷锟窖斤拷液锟斤拷锟斤拷_锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟侥碉拷锟阶結(ji锟斤拷)锟较猴拷,锟斤拷锟斤拷锟絚Protein A锟斤拷G锟斤拷锟斤拷锟脚悸(li锟斤拷n)锟斤拷agaose锟斤拷Sepharose beads锟斤拷锟斤拷,锟斤拷通锟絕锟絰锟侥得碉拷beads锟斤拷protein A锟斤拷G锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷w锟斤拷目锟侥碉拷锟阶復猴拷锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷(j锟斤拷ng)洗锟斤拷锟斤拷貞锟斤拷锟斤拷泳锟较樣撅拷锟絖液,锟斤拷锟斤拷锟5锟斤拷10 min,锟斤拷锟节高溫硷拷還原锟斤拷锟斤拷锟斤拷锟斤拷锟铰ｏ拷锟斤拷原锟絚锟斤拷锟絯锟斤拷锟絰,锟斤拷锟絰锟斤拷锟秸硷拷锟斤拷锟藉，锟斤拷锟斤拷锟叫帮拷锟斤拷锟斤拷锟絯,锟斤拷目锟侥碉拷锟阶猴拷锟斤拷锟斤拷锟斤拷锟絪锟斤拷锟斤拷,锟斤拷
锟斤拷锟竭癸拷锟斤拷锟斤拷锟斤拷Co-Immunoprecipitation锟斤拷Co-IP锟斤拷锟斤拷锟皆匡拷锟絯锟酵匡拷原之锟絞锟侥岋拷一锟斤拷锟斤拷锟矫烇拷锟斤拷A锟斤拷锟斤拷锟斤拷锟叫撅拷锟斤拷锟斤拷锟絴(zh锟斤拷)锟洁互锟斤拷锟矫的斤拷(j锟斤拷ng)锟戒方锟斤拷,锟斤拷锟角確锟斤拷锟缴種锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷(n锟斤拷i)锟斤拷锟斤拷锟斤拷锟洁互锟斤拷锟矫碉拷锟斤拷效锟斤拷锟斤拷,锟斤拷锟斤拷原锟斤拷锟角ｏ拷锟斤拷锟斤拷锟斤拷锟节凤拷變锟皆條锟斤拷锟铰憋拷锟窖斤拷r锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷(n锟斤拷i)锟斤拷锟节碉拷锟絊锟洁蛋锟斤拷锟絴(zh锟斤拷)锟斤拷锟斤拷锟斤拷锟絴(zh锟斤拷)锟絞锟斤拷锟洁互锟斤拷锟矫憋拷锟斤拷锟斤拷锟斤拷锟铰侊拷,锟斤拷锟斤拷锟斤拷玫锟斤拷锟斤拷|(zh锟斤拷)X锟侥匡拷锟絯锟斤拷锟竭筹拷锟斤拷X,锟斤拷锟斤拷么锟絚X锟斤拷锟絯锟斤拷(n锟斤拷i)锟結(ji锟斤拷)锟较的碉拷锟斤拷锟絴(zh锟斤拷)Y也锟杰筹拷锟斤拷锟铰侊拷锟斤拷锟紷锟絅锟斤拷锟斤拷锟斤拷锟斤拷锟节測锟斤拷锟缴種目锟剿碉拷锟斤拷锟絴(zh锟斤拷)锟角凤拷锟斤拷锟絯锟斤拷(n锟斤拷i)锟結(ji锟斤拷)锟斤拷,锟斤拷也锟斤拷锟斤拷锟节確锟斤拷一锟絅锟截讹拷锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷锟铰碉拷锟斤拷锟矫达拷n,锟斤拷
锟斤拷锟斤拷要锟斤拷
锟结供锟斤拷锟絩锟接憋拷锟斤拷锟较猴拷IP锟斤拷WB锟斤拷一锟斤拷锟斤拷锟斤拷锟斤拷锟絑锟斤拷锟絖锟斤拷購锟斤拷
注锟斤拷锟斤拷锟竭癸拷锟斤拷锟斤拷(co-IP)通锟斤拷锟斤拷锟绞癸拷锟轿达拷锟(j锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷r锟斤拷锟阶橈拷品锟斤拷锟斤拷通锟斤拷锟斤拷锟竭筹拷锟斤拷锟絤然锟斤拷锟斤拷使锟矫冿拷锟斤拷牡锟斤拷讟锟狡,锟斤拷锟斤拷也锟斤拷锟斤拷锟斤拷锟絩锟侥碉拷锟阶橈拷品锟斤拷锟,锟斤拷
锟斤拷锟秸伙拷锟斤拷锟斤拷锟斤拷
1. 锟窖斤拷锟狡凤拷锟斤拷蕚涞帮拷讟锟狡,锟斤拷
2. 锟绞備抗锟絯-Protein A/G beads,锟斤拷
3. 锟斤拷品锟斤拷锟斤拷锟絯-Protein A/G bends锟斤拷锟斤拷锟斤拷,锟斤拷
4. 洗脫,锟斤拷
5. WB锟絲锟統锟斤拷
锟斤拷锟秸堬拷锟斤拷(n锟斤拷i)锟斤拷
WB锟絲片,锟斤拷PVDF膜,锟斤拷锟酵岋拷锟斤拷妗
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