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锟斤拷(ji锟斤拷n)锟斤拷
锟絃(zh锟斤拷ng)鏈非撅拷锟絘RNA(long noncoding RNA, lncRNA)指锟斤拷锟斤拷锟絃(zh锟斤拷ng)锟饺筹拷锟絕(gu锟斤拷)200nt锟斤拷RNA锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絘锟斤拷锟阶ｏ拷位锟节硷拷(x锟斤拷)锟斤拷锟剿伙拷锟斤拷|(zh锟斤拷)锟斤拷(n锟斤拷i),锟斤拷锟斤拷锟叫憋拷锟截的讹拷锟斤拷(j锟斤拷)锟結(ji锟斤拷)锟斤拷(g锟斤拷u),锟斤拷锟斤拷锟絚锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷DNA锟斤拷RNA锟洁互锟斤拷锟矫ｏ拷通锟絕(gu锟斤拷)锟斤拷N锟紺(j锟斤拷)锟狡ｏ拷锟斤拷锟斤拷锟接∮,锟斤拷染色锟絴(zh锟斤拷)锟斤拷锟斤拷,锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷锟斤拷锟絳(di锟斤拷o)锟截★拷锟斤拷锟斤拷锟絳(di锟斤拷o)锟斤拷,锟斤拷mRNA锟斤拷锟斤拷头锟斤拷g锟絳(di锟斤拷o)锟截等ｏ拷锟节讹拷N锟斤拷锟斤拷锟较ｏ拷锟斤拷锟斤拷^锟絲锟斤拷锟絎(xu锟斤拷),锟斤拷锟紻(zhu锟斤拷n)锟斤拷{(di锟斤拷o)锟截硷拷锟紻(zhu锟斤拷n)錄猴拷锟絳(di锟斤拷o)锟截等ｏ拷锟絳(di锟斤拷o)锟截伙拷锟斤拷谋锟斤拷_(d锟斤拷)水平锟斤拷
lncRNA锟斤拷锟絚锟斤拷N锟斤拷锟斤拷W(xu锟斤拷)锟絕(gu锟斤拷)锟教碉拷锟絳(di锟斤拷o)锟斤拷,锟斤拷锟节讹拷N锟斤拷锟斤拷锟斤拷锟(d锟斤拷ng)锟叫發(f锟斤拷)锟絔锟斤拷要锟斤拷锟斤拷,锟斤拷該锟絀(l锟斤拷ng)锟斤拷锟斤拷P(gu锟斤拷n)注锟斤拷也锟斤拷锟斤拷锟斤拷锟竭ｏ拷锟窖成烇拷锟絕锟斤拷使RNA(mRNA),锟斤拷小RNA(microRNA,锟斤拷siRNA锟斤拷)之锟斤拷锟杰伙拷锟斤拷M锟絎(xu锟斤拷)锟角筹拷锟斤拷锟斤拷锟叫撅拷锟絀(l锟斤拷ng)锟斤拷,锟斤拷
锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1.锟结供锟斤拷锟統(c锟斤拷)锟斤拷锟斤拷锟斤拷锟叫伙拷锟斤拷號(h锟斤拷o),锟斤拷
2.锟结供锟斤拷锟絩锟津保达拷锟斤拷玫募锟(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷5锟斤拷10锟斤拷6锟轿凤拷锟斤拷锟斤拷锟組锟斤拷(锟斤拷锟斤拷50mg),锟斤拷血液(300锟斤拷l)锟饺樣憋拷锟斤拷锟斤拷,锟斤拷锟津純伙拷锟矫的匡拷RNA
锟斤拷OD260/OD280锟斤拷 1.9-2.1之锟絞锟斤拷锟斤拷锟斤拷锟皆好ｏ拷锟斤拷DNA锟斤拷OD260/OD280锟斤拷1.7-1.9之锟絞,锟斤拷锟斤拷锟斤拷锟皆好ｏ拷,锟斤拷锟斤拷锟紻(zhu锟斤拷n)錄好碉拷cDNA锟斤拷锟斤拷锟斤拷30锟斤拷l锟斤拷锟斤拷锟紻(zhu锟斤拷n)錄的匡拷RNA锟斤拷锟斤拷 锟皆好ｏ拷锟斤拷锟斤拷锟斤拷1.9-2.1之锟絞锟斤拷,锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1.锟结供锟斤拷要锟絲锟統(c锟斤拷)锟斤拷lncRNA锟斤拷锟斤拷锟斤拷锟絈(ch锟斤拷ng),锟斤拷锟斤拷锟教(h锟斤拷o)锟斤拷锟斤拷锟叫ｏ拷
2.锟絖锟絁(r锟斤拷n)锟接憋拷锟斤拷(sh锟斤拷)锟斤拷,锟斤拷锟絅锟斤拷,锟斤拷锟接憋拷锟(l锟斤拷i)锟酵ｏ拷
3.锟斤拷訂锟斤拷同,锟斤拷支锟斤拷锟紸(y锟斤拷)锟斤拷锟斤拷,锟斤拷
4.锟结供锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷品锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷锟組锟斤拷,锟斤拷血液锟斤拷RNA/cDNA锟斤拷,锟斤拷
5.锟絖(k锟斤拷i)展锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷
6.锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟結(ji锟斤拷)锟斤拷锟斤拷,锟斤拷锟絣(f锟斤拷)锟酵筹拷锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟芥；
7.支锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)尾锟斤拷,锟斤拷
8.锟絣(f锟斤拷)锟斤拷詳锟斤拷(x锟斤拷)锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟斤拷,锟斤拷锟斤拷蓪锟(sh锟斤拷)锟(y锟斤拷n)锟(xi锟斤拷ng)目锟斤拷
锟斤拷(b锟斤拷o)锟斤拷锟(n锟斤拷i)锟斤拷
詳锟斤拷(x锟斤拷)锟侥岋拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷锟紼锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷試锟斤拷,锟斤拷锟絰锟斤拷锟斤拷锟斤拷(bi锟斤拷o)锟斤拷(zh锟斤拷n)锟斤拷锟斤拷(xi锟斤拷n),锟斤拷锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷锟斤拷,锟斤拷
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