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锟斤拷(x锟斤拷)锟斤拷16S rDNA锟斤拷锟絅锟絙锟斤拷
通锟絕DNA锟統锟斤拷?q锟斤拷)锟轿拷锟斤拷锟斤拷M锟斤拷锟斤拷N锟絙锟斤拷锟斤拷锟斤拷锟絅锟絙锟斤拷锟斤拷锟角比傦拷锟統(t锟斤拷ng)锟斤拷锟斤拷锟絙锟斤拷锟斤拷锟斤拷锟組锟斤拷锟絙锟斤拷锟斤拷锟斤拷,锟斤拷DNA锟統锟斤拷锟斤拷賴锟斤拷锟絅锟斤拷锟斤拷锟斤拷锟絚,锟斤拷锟斤拷锟斤拷锟叫撅拷锟絅锟斤拷锟斤拷使锟矫★拷锟饺傦拷锟統(t锟斤拷ng)锟斤拷锟斤拷锟絙锟斤拷锟斤拷锟接匡拷锟斤拷,锟斤拷锟斤拷(zh锟斤拷n)锟絖,锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷司锟结供锟斤拷微锟斤拷锟斤拷锟斤拷N锟絙锟斤拷锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷(x锟斤拷)锟斤拷16srDNA锟絙锟斤拷锟斤拷锟斤拷锟絀TS锟絙锟斤拷锟斤拷通锟絕PCR锟経锟斤拷16SrDNA,锟斤拷锟統锟斤拷锟斤拷锟紾eneBank锟斤拷锟斤拷Eztaxon锟斤拷锟饺硷拷锟斤拷,锟斤拷一锟斤拷锟斤拷锟斤拷锟斤拷锟狡讹拷锟斤拷97%锟斤拷锟较就匡拷锟斤拷锟絁(r锟斤拷n)锟斤拷锟斤拷同锟絅锟斤拷锟絅锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)通锟絕锟斤拷锟斤拷锟斤拷驗優(y锟斤拷u)锟斤拷锟斤拷通锟斤拷锟斤拷锟斤拷锟絤锟斤拷锟斤拷99%锟斤拷锟较碉拷微锟斤拷锟斤拷,锟斤拷锟絙锟斤拷锟缴癸拷锟斤拷锟斤拷98%锟斤拷锟斤拷,锟斤拷
锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1.锟斤拷品锟斤拷式锟斤拷锟斤拷锟斤拷锟斤拷锟絩锟斤拷液锟斤拷斜锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷,锟斤拷平锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷取锟矫碉拷DNA,锟斤拷
2.锟斤拷锟斤拷锟狡凤拷锟斤拷锟斤拷MDNA锟斤拷DNA锟斤拷锟>10ng/ul,锟斤拷锟絯锟絜锟斤拷锟斤拷20ul,锟斤拷
3.锟絙锟斤拷锟結(ji锟斤拷)锟斤拷一锟斤拷锟斤拷锟斤拷b锟斤拷锟斤拷锟絅锟斤拷锟斤拷锟絜锟斤拷锟絅锟斤拷锟斤拷微锟斤拷锟斤數(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷锟斤拷锟絇(gu锟斤拷n)锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)缺锟斤拷,锟斤拷只锟斤拷锟絙锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟紷锟絅锟斤拷r锟斤拷锟斤拷锟斤拷锟斤拷锟組,锟斤拷
4.锟斤拷锟斤拷y锟斤拷Y(ji锟斤拷)锟斤拷锟斤拷锟紽(xi锟斤拷n)锟斤拷锟斤拷锟阶凤拷,锟斤拷視锟斤拷锟狡凤拷锟斤拷锟斤拷锟斤拷锟斤拷锟剿撅拷锟斤拷锟饺∪拷~锟斤拷锟斤拷M锟斤拷,锟斤拷锟斤拷锟斤拷锟絟锟斤拷隆锟斤拷锟斤拷锟統锟斤拷锟絙锟斤拷,锟斤拷
5.锟斤拷锟斤拷某些锟铰诧拷锟皆撅拷锟絯锟斤拷請?zh锟斤拷)锟角帮拷f锟斤拷,锟斤拷注锟解保锟給锟斤拷锟斤拷锟剿員锟斤拷锟斤拷锟斤拷锟絟(hu锟斤拷n)锟斤拷锟絣(w锟斤拷i)锟斤拷,锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1.锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟組锟斤拷锟斤拷锟斤拷锟絆(sh锟斤拷)計锟较成ｏ拷
2.PCR锟経锟斤拷16S rDNA,锟斤拷
3.PCR锟絘(ch锟斤拷n)锟斤純锟斤拷,锟斤拷
4.锟統锟斤拷,锟斤拷锟斤拷锟叫比岋拷锟斤拷锟斤拷锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷(n锟斤拷i)锟斤拷
锟斤拷锟斤拷锟斤拷锟斤拷PCR锟絘(ch锟斤拷n)锟斤拷锟斤拷片,锟斤拷NCBI锟斤拷锟叫比岋拷锟絙锟斤拷锟結(ji锟斤拷)锟斤拷,锟斤拷锟統锟斤拷锟芥、锟斤拷锟叫堿锟斤拷锟紻,锟斤拷
注锟斤拷锟斤拷锟斤拷锟絙锟斤拷锟侥撅拷锟斤拷,锟斤拷锟揭傦拷锟斤拷锟斤拷锟斤拷菁锟斤拷锟斤拷锟斤拷锟秸堬拷蜋z之前锟斤拷锟矫憋拷锟絅锟斤拷锟斤拷,锟斤拷
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