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锟斤拷锟斤拷锟絲锟統
锟斤拷锟斤拷锟斤拷位锟斤拷锟斤拷锟斤拷锟斤拷锟饺旧拷w末锟斤拷锟斤拷锟斤拷DNA锟斤拷锟斤拷锟絚锟截讹拷锟斤拷锟斤拷锟絴(zh锟斤拷)锟轿成的忥拷(f锟斤拷)锟斤拷锟絯,锟斤拷锟节硷拷(x锟斤拷)锟斤拷锟斤拷锟斤拷锟絕锟斤拷锟叫憋拷锟斤拷染色锟絯锟斤拷末锟斤拷锟节合猴拷锟絜锟絗锟斤拷锟解，锟皆維锟斤拷染色锟絯锟侥結(ji锟斤拷)锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟絚锟斤拷(w锟斤拷n)锟斤拷,锟斤拷锟斤拷锟斤拷DNA锟缴达拷锟斤拷锟斤拷锟轿的达拷聯(li锟斤拷n)锟截忥拷(f锟斤拷)锟斤拷锟叫組锟斤拷,锟斤拷锟缴讹拷锟斤拷酶锟较成ｏ拷锟节傦拷锟絯锟斤拷锟斤拷锟絃锟絣(f锟斤拷)锟斤拷锟絕锟斤拷锟叫曪拷锟絣(f锟斤拷)锟斤拷锟絃锟饺革拷變,锟斤拷锟截忥拷(f锟斤拷)锟斤拷锟叫革拷變锟斤拷锟斤拷锟脚碉拷變锟斤拷,锟斤拷
锟斤拷锟斤拷锟絃锟斤拷锟角凤拷映锟斤拷(x锟斤拷)锟斤拷锟斤拷B(t锟斤拷i)锟斤拷一锟斤拷锟揭革拷锟(bi锟斤拷o)锟斤拷锟絲锟統锟斤拷锟斤拷锟絃锟饺的凤拷锟斤拷锟叫讹拷N,锟斤拷锟斤拷锟斤拷Southern Blot锟斤拷锟筋常锟矫的凤拷锟斤拷,锟斤拷Southern Blot锟斤拷锟斤拷锟斤拷锟姐、锟缴憋拷锟絕锟斤拷,锟斤拷锟杰猴拷直锟絕锟侥凤拷锟斤拷(y锟斤拷ng)锟斤拷锟斤拷锟絃锟斤拷,锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷Southern Blot平锟絖锟杰烇拷锟斤拷锟结供****锟侥讹拷锟斤拷锟絲锟統锟斤拷锟斤拷(w锟斤拷),锟斤拷
锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1锟斤拷锟斤拷锟絪锟斤拷目锟斤拷(bi锟斤拷o)锟斤拷锟斤拷锟斤拷锟叫和猴拷锟叫达拷锟絪锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷,锟斤拷锟揭傦拷锟斤拷锟斤拷锟斤拷(j锟斤拷)Southern blot锟斤拷锟揭拷锟斤拷锟斤拷锟斤拷x锟斤拷全锟絃锟津部分區(q锟斤拷)锟斤拷锟斤拷锟斤拷探锟斤拷锟斤拷锟,锟斤拷使锟斤拷PCR锟斤拷锟斤拷(bi锟斤拷o)記锟截革拷锟斤拷探針★拷
2,锟斤拷锟斤拷锟絪锟斤拷锟侥革拷锟絴(zh锟斤拷)锟斤拷DNA锟斤拷品锟絴(zh锟斤拷)锟斤拷锟斤拷锟絇(gu锟斤拷n)锟斤拷要,锟斤拷锟絈锟斤拷锟斤拷锟侥成旓拷锟斤拷請锟絖锟斤拷DNA锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷,锟斤拷锟組锟斤拷锟斤拷锟斤拷锟組(j锟斤拷n)锟斤拷試锟斤拷锟斤拷锟斤拷取锟斤拷委锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷取,锟斤拷
A锟斤拷锟斤拷锟斤拷MDNA锟絲锟統锟斤拷OD260/OD280=1.8-2.0,锟斤拷锟斤拷却锟斤拷锟0.3锟斤拷g/ul,锟斤拷锟絪锟斤拷锟斤拷模锟斤拷DNA锟絪60 锟斤拷g/泳锟斤拷/锟轿ｏ拷
B,锟斤拷請?zh锟斤拷)峁╋拷锟斤拷锟組DNA锟絲锟統锟紻片,锟斤拷要锟斤拷o锟斤拷锟解，锟給锟斤拷锟斤拷锟斤拷染,锟斤拷
C,锟斤拷锟絲锟統锟斤拷品锟酵滐拷锟絓輸锟斤拷锟斤拷司锟斤拷
3,锟斤拷锟酵戯拷锟斤拷锟斤拷o锟斤拷锟结供锟斤拷品锟絴(zh锟斤拷)锟侥伙拷锟斤拷MDNA锟斤拷品,锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷锟皆庯拷锟斤拷锟狡傦拷锟斤拷锟斤拷锟絊outhern Blot锟絲锟統锟斤拷DNA锟斤拷品锟斤拷锟酵戯拷锟结供锟斤拷品要锟斤拷锟斤拷锟铰ｏ拷
A,锟斤拷锟斤拷液锟斤拷锟斤拷锟斤拷锟斤拷500ul,锟斤拷锟斤拷锟斤拷锟斤拷锟竭干憋拷锟絓輸
B锟斤拷锟組锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷小锟斤拷2g,锟缴憋拷锟絓輸
C,锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷锟结供锟斤拷锟絩锟侥硷拷(x锟斤拷)锟斤拷锟斤拷锟斤拷,锟斤拷锟組锟斤拷锟斤拷要使锟斤拷锟絵锟斤拷液锟絖洗
4锟斤拷請注锟斤拷锟斤拷锟斤拷MDNA酶锟叫凤拷锟斤拷,锟斤拷锟斤拷锟斤拷目锟斤拷锟斤拷要锟矫碉拷锟角筹拷規(gu锟斤拷)锟斤拷锟斤拷锟斤拷锟皆冿拷(n锟斤拷i)锟斤拷酶,锟斤拷锟斤拷锟絶锟斤拷锟斤拷锟矫革拷锟斤拷M锟矫伙拷锟叫客戯拷锟结供锟斤拷锟斤拷(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟皆冿拷(n锟斤拷i)锟斤拷酶锟斤拷
锟斤拷锟斤拷锟絲锟統锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷
1.锟斤拷锟斤拷MDNA锟斤拷取
2.锟斤拷锟斤拷MDNA酶锟斤拷锟斤拷锟斤拷
3.DNA锟斤拷锟斤拷酶锟斤拷锟斤拷锟斤拷锟斤拷a(ch锟斤拷n)锟斤拷
4.锟斤拷脂锟斤拷锟斤拷锟絲锟接
5.锟紻(zhu锟斤拷n)膜
6.锟教讹拷
7. DIG探針橈拷(bi锟斤拷o)記,锟斤拷
8. Southern锟絪锟斤拷,锟斤拷
8. 锟截癸拷锟紷色锟斤拷
9. 锟结交锟斤拷影锟絲锟統锟斤拷锟,锟斤拷锟斤拷锟斤拷詳锟斤拷(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷蛯锟斤拷Y(ji锟斤拷)锟斤拷锟斤拷,锟斤拷
Southern Blot锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟侥硷拷
锟斤拷锟絑锟斤拷锟絖(d锟斤拷)負(f锟斤拷)責(z锟斤拷)锟斤拷锟酵戯拷锟结供锟斤拷品锟組(j锟斤拷n)锟斤拷DIG探針橈拷(bi锟斤拷o)記锟絚Southern锟絪锟斤拷锟絲锟統锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷K锟结供锟絲锟統锟斤拷婕帮拷z锟統锟紻片
1锟斤拷DNA锟斤拷品锟絲锟統锟斤拷妫拷锟斤拷锟狡凤拷锟斤拷锟竭,锟斤拷請注锟解保锟斤拷锟捷ｏ拷
2,锟斤拷锟斤拷(bi锟斤拷o)锟斤拷(zh锟斤拷n)锟斤拷锟斤拷锟斤拷虉锟斤拷
3锟斤拷southern blot锟斤拷锟結(ji锟斤拷)锟斤拷锟絲锟統锟斤拷锟
4,锟斤拷锟斤拷锟斤拷菍锟斤拷锟斤拷锟絶锟斤拷锟揭拷锟,锟斤拷請?zh锟斤拷)锟角帮拷锟诫娀锟斤拷]锟斤拷锟絝锟斤拷
锟斤拷驗范锟斤拷锟紻片锟斤拷
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